Isothermal titration calorimetry
Isothermal titration calorimetry (ITC) is a powerful technique that determines thermodynamic parameters of ligand-macromolecule interactions. In a typical experiment, the heat of each injection at a fixed temperature (T) as a function of the ligand/macromolecule molar ratio is plotted to yield a binding curve. The binding enthalpy (ΔH) is directly measured. The binding constant (Ka) and the stoichiometry (n) are extrapolated from the binding curve. The binding free energy (ΔG) is calculated based on the equation ΔG = -RTln(Ka). The binding entropy is then calculated using the equation ΔG = ΔH -TΔS. In addition, ITC can be used for determining the changes of heat capacity (ΔCp) and studying chemical (enzymatic) kinetics to measure KM and kcat.
Location
Affinity ITC is located in Classroom Building #113.
Authorized Users
Only authorized users are allowed to operate the ITC instrument independently. Potential users should contact Dr. Liang Xue to schedule training. The training will cover sample preparation, operation and cleaning of instrument, and data analysis. Three experiments will be conducted including the titration of Ca(II) to EDTA (standard), buffer to buffer, and an actual sample provided by the user. In order to better maintain the instrument, only limited authorized users from chemistry and pharmacy will be assigned.
Scheduling
Authorized users will be invited to join the ITC Google Calendar. To reserve time slots, please type in your name and lab.
Instrument maintenance and operation cost
- The routine maintenance will be performed by Dr. Liang Xue.
- Users are expected to provide all consumables specific to their experiment.
- Users are responsible for cleaning the instrument based on the instruction after each use.
- Users should clean the working area after each use.
- After their experiment, users should fill out the sign-in sheet next to the instrument. In the sign-in sheet, please provide the operation hours of the experiment. The time will be used for calculation of split cost for repairs/parts/services resulted from normal wear.
- Users are responsible for repairs or parts due to damage caused by their carelessness or neglect. This includes replacing the broken titration syringe or the needle bent.
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